Diterpene Discovered Using Isotope Labeling

  • Author: ChemistryViews.org
  • Published: 20 November 2017
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA
  • Source / Publisher: Beilstein Journal of Organic Chemistry/Beilstein Institute
thumbnail image: Diterpene Discovered Using Isotope Labeling

Herpetosiphon aurantiacus 114-95T is a predatory bacterium. Its genome contains instructions for terpene cyclases, enzymes that are involved in the synthesis of terpenes. The products of several of these enzymes had not been identified so far.


Markus Nett, Technical University Dortmund, Germany, and colleagues have discovered a new natural product, herpetopanone (pictured), which is produced by Herpetosiphon aurantiacus 114-95T. The team fed glucose, labeled with 13C atoms, to a culture of the bacterium, and used liquid chromatography–mass spectrometry (LC-MS) to analyze the metabolic products of the bacteria. They compared the isotope patterns of these spectra to the spectra of the metabolites produced from unlabeled glucose and were able to identify herpetopanone, a diterpene with the formula C20H36O4.


The researchers repeated the fermentation on a larger scale and were able to extract 19 g of herpetopanone. The product was characterized using 1H NMR spectroscopy. According to the team, its structure is unprecedented in bacteria.


 

Article Views: 516

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

Bookmark and Share

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission. more


CONNECT:

ChemistryViews.org on Facebook

ChemistryViews.org on Twitter ChemistryViews.org on YouTube ChemistryViews.org on LinkedIn Sign up for our free newsletter


A product of ChemPubSoc Europe (16 European Chemical Societies)and Wiley-VCH