Feeding Fruit Flies So They Fluoresce

  • Author: ChemistryViews
  • Published: 22 October 2011
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
  • Source / Publisher: Small/Wiley-VCH
thumbnail image: Feeding Fruit Flies So They Fluoresce

The fruit fly, Drosophila melanogaster, is one of the most prominent models to study developmental biology. Bioimaging of flies can be difficult — the handling of flies inside an NMR tube has been described as a very tricky job. Another option is optical imaging using fluorescent nanomaterials as contrast agents. These need to be introduced by intravenous/intramuscular injection or by gene translation, both of which can be difficult and time consuming.


Sabyasachi Sarkar and colleagues, Indian Institute of Technology, Kanpur, have developed a much simpler way to introduce fluorescent nanomaterials into fruit flies. They use water-soluble carbon nano-onions made from wood waste. The nano-onions are added to the fruit flies’ food and ingested orally by the flies. Just 4 ppm of nano-onions allowed the optical fluorescence microscopy imaging of all the stages of the fruit fly life cycle. The fluorescent fruit flies showed no toxic effects — upon withdrawal of the nano-onions from the food, they excreted the fluorescing material and continued to proliferate to the next generation, demonstrating a return to their normal lives.

This technique may be used in the easy, noninvasive image-based studies of other animals, and could ultimately be extended to human beings.

Image: © Wiley-VCH


Article Views: 2378

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

Bookmark and Share

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission. more


CONNECT:

ChemistryViews.org on Facebook

ChemistryViews.org on Twitter ChemistryViews.org on YouTube ChemistryViews.org on LinkedIn Sign up for our free newsletter


A product of ChemPubSoc Europe (16 European Chemical Societies)and Wiley-VCH