Angling for a Route to Fish Proteins

  • Author: Vikki Cantrill
  • Published: 26 June 2013
  • Copyright: WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
  • Source / Publisher: Organic & Biomolecular Chemistry/RSC
  • Associated Societies: Royal Society of Chemistry (RSC), UK
thumbnail image: Angling for a Route to Fish Proteins

Antarctic fishes survive in freezing seawater by producing protective antifreeze proteins. One such protein is antifreeze potentiating protein (AFPP), which is considered an adjunct to the known antifreeze glycoproteins that circulate in the blood. To understand the functional properties of AFPP, large quantities of this scarce product are needed, so a formal chemical synthesis is required.


AFPP is a large protein (15.5 kDa) that binds to ice crystals and inhibits their growth. Owing to its size, Margaret Brimble and colleagues from the University of Auckland, New Zealand, and the University of Illinois at Urbana-Champaign, USA, attempted the synthesis of AFPP by means of a convergent ligation strategy — an approach that synthesizes manageable fragments individually, then joins them together to make a much larger molecule.


Although lack of solubility was a challenge, the four separate fragments were successfully synthesized by using standard peptide bond forming techniques, and then stitched together to form a 132 amino acid protein, a masked analogue of AFPP. The key step was the introduction of a solubilizing tag (a pentalysine), which improved handling and purification.


Article Views: 2007

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

Bookmark and Share

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission. more


CONNECT:

ChemistryViews.org on facebook

ChemistryViews.org on twitter ChemistryViews.org on YouTube ChemistryViews.org on LinkedIn Sign up for free newsletter



A product of ChemPubSoc Europe (16 European Chemical Societies)and Wiley-VCH