DNAzyme-Based Paper Sensor for Bacteria

  • ChemPubSoc Europe Logo
  • Author: ChemBioChem
  • Published Date: 27 January 2019
  • Source / Publisher: ChemBioChem/Wiley-VCH
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA
thumbnail image: DNAzyme-Based Paper Sensor for Bacteria

Related Societies

Bacterial infections can pose a serious public health threat. The successful treatment of such—potentially deadly—infections largely depends on the rapid and convenient detection of the associated bacteria. Bacteria multiply quickly, which can lead to sepsis. Unfortunately, the efficient and simple detection of bacteria still is a major challenge. It sometimes takes several days to confirm a diagnosis with the tools currently available to doctors.


M. Monsur Ali, McMaster University, Canada, Weian Zhao, University of California, Irvine, USA, and colleagues have developed a DNA-catalyst-based simple fluorescent assay printed on paper, which can be used to screen multiple samples for deadly bacteria. The DNA catalyst (also called DNAzyme) specifically senses the presence of a particular pathogen, Klebsiella pneumoniae, which has been identified as a "priority pathogen" by the World Health Organization (WHO) due to antibiotic resistances and the high prevalence of infections.


The inactive DNAzyme contains a component that can act as a fluorescent dye (fluorescein-dT) and another component that acts as a quencher (dabcyl-dT). It has only a low fluorescence due to the presence of the quencher close to the fluorophore. The DNAzyme is activated upon binding to a target molecule which is present in cell lysates (i.e., the contents of broken-down cells) of K. pneumoniae. It then cleaves the bond to the quencher, which causes an enhanced fluorescence signal. The signal (pictured right, control on the left) can be easily visualized by a fluorescence scanner or even a cell phone.


The assay was performed on paper with circular reaction spots, which are surrounded by wax. The DNAzyme was applied to the spots and dried overnight. The test requires only a small sample volume and the results can be obtained within 30 minutes. The DNAzyme is moderately specific. The researchers believe that an assay with improved specificity could allow the fast screening of a large number of samples and eventually help doctors to decide on the appropriate therapeutic intervention.


 

Article Views: 1191

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

Bookmark and Share

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission. more


CONNECT:

ChemistryViews.org on Facebook

ChemistryViews.org on Twitter ChemistryViews.org on YouTube ChemistryViews.org on LinkedIn Sign up for our free newsletter


A product of ChemPubSoc Europe (16 European Chemical Societies)and Wiley-VCH