Isotopically Labelled Clickable Glutathione

  • Author: ChemBioChem
  • Published Date: 04 November 2019
  • Source / Publisher: ChemBioChem/Wiley-VCH
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA
thumbnail image: Isotopically Labelled Clickable Glutathione

Related Societies

The oxidation of cysteine residues in proteins with glutathione (S-glutathionylation) in the presence of hydrogen peroxide controls many complex biological processes in cells. Therefore, the identification of specific target proteins susceptible to S-glutathionylation is important to understand the biological consequences of oxidative conditions. However, methods to identify S-glutathionylation at the proteomic level are scarce.

Young-Hoon Ahn, Wayne State University, Detroit, MI, USA, and colleagues have developed a mass-spectrometric method to identify and quantify target proteins of S-glutathionylation. The team used cells with a genetically engineered glutathione synthetase enzyme to make isotopically labeled glutathione derivatives with a "clickable" azide functional group (pictured). These two otherwise identical glutathione derivatives have a small mass difference. A click reaction with an alkyne-functionalized reagent enabled the team to label the glutathionylated proteins and to separate and identify them.

The researchers used this approach to find proteins that are susceptible to S-glutathionylation in cardiomyocytes (heart muscle cells). The team used two different batches of the cells and reacted them with either the heavy or the light glutathione derivative. The glutathionylation was induced by the addition of H2O2 and the modified peptides were labeled and extracted. Then, using mass spectrometry (MS), the team looked for pairs of peptides with exactly the same mass difference as the two glutathione derivatives and quantified them. They found elevated levels of S-glutathionylation on 797 cysteines. According to the team, this method could be broadly applicable to various biological systems to identify S-glutathionylation.



Article Views: 1423

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

If you would like to reuse any content, in print or online, from, please contact us first for permission. more

Follow on Facebook Follow on Twitter Follow on YouTube Follow on LinkedIn Follow on Instagram RSS Sign up for newsletters

Magazine of Chemistry Europe (16 European Chemical Societies) published by Wiley-VCH