Activity of Xeno-Nucleic-Acid (XNA) Enzymes

  • Author: ChemBioChem
  • Published Date: 25 November 2019
  • Source / Publisher: ChemBioChem/Wiley-VCH
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA
thumbnail image: Activity of Xeno-Nucleic-Acid (XNA) Enzymes

Related Societies

Artificial genetic polymers, also known as xeno-nucleic acids or XNAs, have unique physical and chemical properties that could allow them to catalyze chemical reactions with high efficiency. However, most of these XNA enzymes (XNAzymes) generated by in-vitro selection have a much lower activity than their "natural" DNA and RNA counterparts.


John C. Chaput, University of California, Irvine, USA, and colleagues have explored the catalytic potential of an XNAzyme that is composed entirely of 2'-fluoroarabino nucleic acid (FANA, pictured right), named FANAzyme 12-7. This enzyme was evolved to cleave RNA at a specific phosphodiester linkage. The team found that the FANAzyme outcompetes equivalent DNAzymes in model reactions performed under a range of conditions, including those that simulate the physiological environment of a cell.


According to the researchers, this is the first example of an in vitro-selected XNA enzyme with catalytic efficiencies greater than a DNAzyme. This might be important for the evolution of XNA catalysts and future applications in synthetic biology.


 

 

Article Views: 1318

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

Bookmark and Share

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission. more


CONNECT:

ChemistryViews.org on Facebook

ChemistryViews.org on Twitter ChemistryViews.org on YouTube ChemistryViews.org on LinkedIn Sign up for our free newsletter


Magazine of Chemistry Europe (16 European Chemical Societies)published by Wiley-VCH