Enzyme-Controlled Drug Delivery

  • ChemPubSoc Europe Logo
  • Author: Claire Cobley
  • Published Date: 13 April 2014
  • Source / Publisher: Chemistry – A European Journal/Wiley-VCH
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
thumbnail image: Enzyme-Controlled Drug Delivery

Related Societies

Gated mesoporous silica nanoparticles are a popular platform for stimulus-induced drug delivery owing to their well-defined porous structure. Many different stimuli have been investigated, including changes in pH or temperature, irradiation with light, and, more recently, interactions with biomolecules.

Ramón Martínez-Máñez and colleagues, Universidad de Valencia, Spain, have developed a way to induce release from mesoporous silica nanoparticles (pictured) with enzymes. ε-Poly-L-lysine, containing 25–35 L-lysine amino acid units linked by ε-amino groups, was attached to the surfaces of the nanoparticles through both specific and nonspecific routes. In the presence of proteases, hydrolysis of the amide bonds occurred, resulting in the release of the cargo, with different delivery profiles that depended on the attachment method.

This strategy was used to deliver cargo to HeLa cells, the most commonly used human cell line. First, a Ru-based dye was loaded to study the distribution of the cargo. After incubation for 48 hours, fluorescence was observed mainly in the lysosomes. HeLa cells were then treated with nanoparticles loaded with the chemotherapeutic agent camptothecin, and dose-dependent cell death occurred.

Article Views: 2760

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

Bookmark and Share

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission. more


ChemistryViews.org on Facebook

ChemistryViews.org on Twitter ChemistryViews.org on YouTube ChemistryViews.org on LinkedIn Sign up for our free newsletter

A product of ChemPubSoc Europe (16 European Chemical Societies)and Wiley-VCH