Photoactivatable Gene Vectors

  • Author: Jonathan Faiz
  • Published Date: 05 July 2015
  • Source / Publisher: Angewandte Chemie International Edition/Wiley-VCH
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
thumbnail image: Photoactivatable Gene Vectors

The development of vectors for gene therapy is often hampered by the fact that nonviral vectors become trapped in endo/lysosomes after endocytosis and the nucleic acids that are being transported end up being destroyed. Bin Liu and colleagues, National University of Singapore and Agency for Science, Technology and Research (A*STAR), Singapore, have come up with a polymeric gene delivery vector that can not only escape from endo/lysosomes, but also unpack DNA in one light excitation process.

The researchers designed a gene delivery vector that contains a photosensitizer, an aminoacrylate linker that can be cleaved by reactive oxygen species (ROS), and poly(ethylene glycol) units for water solubility. In aqueous media, the polymer self-assembles into nanoparticles that show a bright red fluorescence and can bind to DNA by electrostatic interactions. After endocytosis, the nanoparticles are trapped in endo/lysosomes.

Irradiation with light results in two concurrent processes (see picture): the formation of ROS, which destroy the endo/lysosomal membrane, and DNA release upon breaking of the polymer when the ROS-reactive linkers are cleaved. Studies showed that the released DNA accumulated in nuclei, and that the transfection efficiency of the system was 50 % more higher than that of commercially available products. This strategy should be applicable to therapeutic DNA or siRNA for gene therapy.


Article Views: 2752

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

If you would like to reuse any content, in print or online, from, please contact us first for permission. more

Follow on Facebook Follow on Twitter Follow on LinkedIn Follow on Instagram RSS Sign up for newsletters

Magazine of Chemistry Europe (16 European Chemical Societies) published by Wiley-VCH