Uncovering Invisible Biomarkers

  • Author: ChemistryViews
  • Published: 14 November 2011
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
  • Source / Publisher: Journal of the American Chemical Society/ACS Publications
thumbnail image: Uncovering Invisible Biomarkers

Many low-abundance biomarkers for the early detection of cancer and other diseases are invisible to mass spectrometry (MS). They exist in bodily fluids at concentrations below the range of detection by MS, are masked by high-abundance proteins, such as albumin and immunoglobulins, and are very labile.


Alessandra Luchini and co-workers, George Mason University, Virginia, USA, have reported a one-step, in-solution preprocessing step that achieved a 10,000-fold effective amplification of the analyte concentration, enabling MS discovery of candidate biomarkers that were previously undetectable.


The team functionalized core–shell hydrogel nanoparticles with novel organic dye baits. The hydrogel shell had an effective pore size that acted as a molecular sieve with a cutoff determined by the cross-link density. This excluded high-molecular weight proteins such as albumin and immunoglobulins. The shell also contained vinylsulfonic acid to selectively repulse peptides derived from albumin that were small enough to penetrate the shell. Biomarkers small enough to enter the particles were captured by the high-affinity bait.

The nanoparticles protected interleukin-6 from enzymatic degradation in sweat and increased the effective detection sensitivity of human growth hormone in human urine.

Image: © Wiley-VCH


Article Views: 2575

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission. more


CONNECT:

ChemistryViews.org on Facebook

ChemistryViews.org on Twitter ChemistryViews.org on YouTube ChemistryViews.org on LinkedIn Sign up for our free newsletter


Magazine of Chemistry Europe (16 European Chemical Societies)published by Wiley-VCH