Fluorescence-Enhanced Detection of Nucleic Acids

  • ChemPubSoc Europe Logo
  • Author: ChemPlusChem
  • Published Date: 01 January 2012
  • Source / Publisher: ChemPlusChem/Wiley-VCH
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
thumbnail image: Fluorescence-Enhanced Detection of Nucleic Acids

Related Societies

Xuping Sun and co-workers, Chinese Academy of Sciences and King Abdulaziz University, Jeddah, Saudi Arabia, report a new application of zeolitic imidazolate framework-8 nanoparticles (ZIFNPs) as an effective sensing platform for the detection of fluorescence-enhanced nucleic acids. The DNA detection is accomplished in two steps:

1) the ZIFNP adsorbs and quenches the dye-labeled single-stranded DNA probe, and

2) subsequent hybridization of the probe with its target produces a double-stranded DNA, which detaches from the ZIFNP, thus resulting in recovery of dye fluorescence (see figure).


These researchers used ZIFNPs as a sensing platform for fluorescence-enhanced detection of an oligonucleotide sequence associated with human immunodeficiency virus (HIV) as their model system. Overall, the method developed is very sensitive, highly selective down to single-base mismatch, and simple. It may have the potential use for universal and effective fluorescence-enhanced detection sensitive and selective to the target molecule studied such as proteins and heavy metal ions.

Image: © Wiley-VCH


Article Views: 2469

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

Bookmark and Share

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission. more


CONNECT:

ChemistryViews.org on Facebook

ChemistryViews.org on Twitter ChemistryViews.org on YouTube ChemistryViews.org on LinkedIn Sign up for our free newsletter


A product of ChemPubSoc Europe (16 European Chemical Societies)and Wiley-VCH