Synthetic Biology Toolboxes for Cyanobacteria

  • Author: Anar Murphy
  • Published: 17 November 2015
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
  • Source / Publisher: Biotechnology & Bioengineering/John Wiley & Sons
thumbnail image: Synthetic Biology Toolboxes for Cyanobacteria

Cyanobacteria are autotrophic prokaryotes, i.e., organisms that produce complex organic compounds themselves. They have a high potential to become the mainstream biotechnology production platform for high-value bioproducts. However, the synthetic biology tools to predictably control gene expression in cyanobacteria are far behind those developed for other microorganisms.


Brian Pfleger, University of Wisconsin-Madison, Madison, WI, USA, and colleagues built a novel inducible small RNA (sRNA) system to control gene expression in the fast-growing, cyanobacterium Synechococcus sp. strain PCC 7002. For this, they combined a newly constructed anhydrotetracycline (aTc)-based induction system and a trans-acting sRNA system to produce an inducible sRNA system to function in the cyanobacterium strain. Compared to other induction systems in cyanobacteria, the aTc inducible system is more tightly regulated, has significant higher fold induction at non-toxic levels, and shows a very sensitive induction. This makes it advantageous, especially when a lesser quantitiy of protein is desired.

Additionally, the team showed that the native RNA binding Hfq protein – critical for regulation of sRNA systems – was not involved in the function of the adapted sRNA system.The Hfq interruption suppresses the natural competency of the cyanobacterium strain. Further research could faciliate the use of native syanobacterial sRNA scaffolds in synthetic biology projects.


This synthetic biology tool can be used to study the metabolism and generate new industrial strains of cyanobacteria.


 

Article Views: 1642

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

Bookmark and Share

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission. more


CONNECT:

ChemistryViews.org on Facebook

ChemistryViews.org on Twitter ChemistryViews.org on YouTube ChemistryViews.org on LinkedIn Sign up for our free newsletter


A product of ChemPubSoc Europe (16 European Chemical Societies)and Wiley-VCH