Better Characterization of Heterogeneous Biocatalysts

  • ChemPubSoc Europe Logo
  • Author: ChemCatChem
  • Published Date: 23 February 2018
  • Source / Publisher: ChemCatChem/Wiley-VCH
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA
thumbnail image: Better Characterization of Heterogeneous Biocatalysts

Related Societies

Single-particle studies have been used for optimizing the performance of heterogeneous catalysts, but the approach is underused in heterogeneous biocatalysts. This is due to the fact that such systems are still developed through trial-and-error, and their characterization is based on observable parameters at a macroscopic level, which are hardly able to reveal the spatio-temporal performance and the intraparticle environments of the immobilized biocatalysts.


Fernando López-Gallego, CIC biomaGUNE, San Sebastian, Spain, and colleagues highlight the need to develop new techniques to monitor the structure and function of proteins during and after the immobilization process with spatio-temporal resolution at the single-particle level.


Different spectroscopic methods, such as micro-FTIR, micro-Raman, fluorescence, and atomic force spectroscopy, and circular dichroism, as well as microscopic methods including fluorescence, atomic force, and multiphoton laser scanning microscopy, reveal how the material defects, size dispersion, and functional patterning of the solid materials influence the final properties of the immobilized enzymes. Insights into protein conformation, mass transfer effects, and enzyme kinetics at the micro/nanoscale within a single particle contribute to understanding the productivity and stability of the heterogeneous biocatalysts determined by macroscopic analysis.


Although all this information contributes to develop more rational, reliable, and reproducible procedures for the fabrication of heterogeneous biocatalysts, there is still a long way to go until structure-function relationships of immobilized enzymes on porous materials at the nanometric scale can be established. In operando studies at the single-particle level must still gain in temporal resolution to study the use of reactants and the protein conformational changes during the biocatalytic transformation at very short times. Furthermore, there is a need to expand these techniques to monitor enzyme properties in complex chemoenzymatic processes and more sophisticated operational designs such as flow-(micro)reactors.


 

Article Views: 939

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

Bookmark and Share

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission. more


CONNECT:

ChemistryViews.org on Facebook

ChemistryViews.org on Twitter ChemistryViews.org on YouTube ChemistryViews.org on LinkedIn Sign up for our free newsletter


A product of ChemPubSoc Europe (16 European Chemical Societies)and Wiley-VCH