Electrolyte Layers Stabilize Mushroom Enzyme

  • Author: Lois O'Leary
  • Published Date: 14 January 2012
  • Source / Publisher: ChemCatChem/Wiley-VCH
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
thumbnail image: Electrolyte Layers Stabilize Mushroom Enzyme

Related Societies

Catechols are a family of bioactive substances that can be derived from phenols. Their environmentally friendly synthesis is, however, a sticking point in biochemistry. Raffaele Saladino and co-workers, University of Tuscia, Italy, have shown how mushroom tyrosinase, immobilized on a solid support, can catalyze the oxidation of phenols to their corresponding catechols.

After immobilization on Eupergit C 250 L, the mushroom tyrosinase was stabilized further by a coating of polyelectrolyte layers, formed by means of the layer-by-layer technique. The enzyme was suspended first in positively, then in negatively charged electrolyte solutions. This process was repeated twice to build up a three-layer coating around the enzyme. Although slightly decreasing the activity, the polyelectrolyte layers were found to enhance the selectivity and stabilization of the tyrosinase.

The immobilized tyrosinase selectively oxidized para- and meta-substituted phenols; ortho-derivatives were not oxidized. Furthermore, kinetic investigations showed the enzyme to be more reactive in organic than buffer solution. The system, which works at room temperature, retained catalytic activity over successive runs and provides a mild and environmentally friendly route to catechols.

Image: © Wiley-VCH

Article Views: 3762

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission and consult our permission guidance prior to making your request

Follow on Facebook Follow on Twitter Follow on YouTube Follow on LinkedIn Follow on Instagram RSS Sign up for newsletters

Magazine of Chemistry Europe (16 European Chemical Societies) published by Wiley-VCH