Taking up ArMs

Taking up ArMs

Author: Meghan Campbell

Metalloenzymes are known for catalyzing a wide variety of reactions in nature. Synthetic biologists are hoping to repurpose non-metallic proteins by using metal-coordinating amino acids to make artificial metalloenzymes.

This technique, developed by Jared C. Lewis and colleagues, University of Chicago, IL, USA, uses a well-established method of suppressing natural stop codons with a non-canonical amino acid to incorporate the metal-binding site at any site within the synthetic protein. Once incorporated, the metal-binding amino acid (p-azido-L-phenylalanine; Az) can coordinate rhodium ions, creating the first rhodium metalloenzyme.

In addition to creating a dirhodium-containing ArM, the team demonstrated the ability of this enzyme to catalyze a cyclopropanation reaction and to insert Si–H into a carbon scaffold. Although these are just model reactions, they show the potential for these ArMs to become versatile catalysts for a wide variety of important reactions. The design of the ArMs also allows for modular substitution with other metal-coordinating groups, making them flexible for many different applications.

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