Solid-Phase PCR in Real Time

  • Author: ChemistryViews
  • Published: 14 March 2011
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA
  • Source / Publisher: Small/Wiley-VCH
thumbnail image: Solid-Phase PCR in Real Time

The polymerase chain reaction (PCR) has become the standard tool in molecular biology to discrimination between one or more DNA sequences of interest. Solid-phase PCR holds additional promise for high-throughput DNA sequencing and large-scale single-nucleotide polymorphism analysis. However, it has relatively low yields of amplicon and subsequent low efficiencies which make real-time (RT) monitoring difficult.

Jeroen Lammertyn and colleagues, Katholieke Universiteit Leuven, Belgium, have combined solid-phase PCR with surface plasmon resonance (SPR) to give real-time monitoring of the PCR. The fiber optic-based SPR platform comprised an SPR sensor with gold-nanobead signal enhancement that permitted remote SPR sensing inside a standard PCR thermocycler. The SPR system could monitor RT-PCR in nontransparent media and differentiate products in single-nucleotide polymorphism screenings using high-resolution melting data, making it ideal for food and medical diagnostics.

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Magazine of Chemistry Europe (16 European Chemical Societies) published by Wiley-VCH