Synthetic Cells for Tumor Treatment

  • Author: Lisa-Marie Rauschendorfer
  • Published: 19 May 2018
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA
  • Source / Publisher: Advanced Healthcare Materials/Wiley-VCH
thumbnail image: Synthetic Cells for Tumor Treatment

Synthetic cells are artificial particles that can synthesize proteins from a DNA template autonomously. They contain the molecular machinery necessary for protein synthesis and need to be preloaded with the required template DNA, amino acids, and energy-supplying molecules. This cell-free protein synthesis (CFPS) offers a wide variety of applications because it can overcome the limitations of biological protein expressions. Even cytotoxic proteins can be synthesized. These proteins usually kill their host cell system before a significant level of expression is reached.

Avi Schroeder, Israel Institute of Technology, Haifa, and colleagues have developed a targeted drug-delivery system for the treatment of tumors that uses CFPS. Biologically active, cytotoxic proteins are produced by CFPS and released directly at the site of the tumor. The team encapsulated an Escherichia coli-based CFPS system inside lipid-based vesicles. An aqueous solution containing the CFPS system was pipetted into a mineral oil containing a vesicle‐forming lipid, 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine (POPC), and cholesterol. Through several cycles of centrifugation, liposomes (vesicles) containing the CFPS system were formed.

This platform was used to synthesize a variety of proteins with molecular weights of up to 66 kDa and also successfully used to secrete Pseudomonas exotoxin A. The effectiveness of this therapeutic protein was shown in vitro as well as in vivo: when locally injected into a tumor in mice, the histological analysis indicates a robust apoptosis (death) of cancer cells.


Article Views: 2380

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

If you would like to reuse any content, in print or online, from, please contact us first for permission. more

Follow on Facebook Follow on Twitter Follow on YouTube Follow on LinkedIn Follow on Instagram RSS Sign up for newsletters

Magazine of Chemistry Europe (16 European Chemical Societies) published by Wiley-VCH