Optogenetics – Revolutionizing Biochemistry

  • Author: ChemistryViews
  • Published: 17 January 2013
  • Copyright: WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
  • Source / Publisher: Biology of the Cell/Societe Francaise des Microscopies and Societe de Biologie Cellulaire de France
thumbnail image: Optogenetics – Revolutionizing Biochemistry

Optogenetic technologies combine technologies from optics and genetics to use light to control cell behaviors and molecular processes in real-time.
Optogenetic reporters are one of the most important tools of optogenetics. Most of these indicator proteins are composed of naturally evolved fluorescent proteins that have been tuned by protein engineering in conjunction with a variety of other proteins that evolved to sense biological parameters such as ion concentrations or membrane potential. These biosensors are genetically encoded, which means that living cells can be instructed by genetic modification to synthesize these fluorescent proteins. The cell's fluorescence then indicates the biological parameter of interest.

Thomas Knöpfel, RIKEN Brain Science Institute, Japan, and colleagues from the University of Alberta, Edmonton, Canada, systematize the established transduction mechanisms of these engineered proteins. They also outline some of the challenges that need to be overcome to move this field of research forward. One of the most pressing challenges is the development of proteins with stable bright far-red or infra-red fluorescence, long wavelength light that penetrates tissue very well.

Article Views: 5067

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in


maraim ibrahim wrote:


To use optic light as determined method is new technology in science and I am very interested to learn more about this technology

Tue Jul 03 10:04:13 UTC 2018

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission and consult our permission guidance prior to making your request

Follow on Facebook Follow on Twitter Follow on YouTube Follow on LinkedIn Follow on Instagram RSS Sign up for newsletters

Magazine of Chemistry Europe (16 European Chemical Societies) published by Wiley-VCH