E. coli that Smells Like Lemon

  • Author: Lisa-Marie Rauschendorfer
  • Published: 20 July 2014
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA
  • Source / Publisher: Biotechnology Journal/Wiley-VCH
thumbnail image: <i>E. coli</i> that Smells Like Lemon

The cyclic monoterpene limonene is naturally found in the zests of lemons and is extensively used in fragrance, food and cosmetic industry. It also is gaining attention as jet fuel of biological origin. Genetically engineered Escherichia coli cells, also called cellular biocatalysts, produce limonene from the cheap and renewable resources glycerol and glucose by a fermentative process. This is achieved by the biotechnological introduction of enzymes into E. coli that are not a natural part of this bacterium but are able to catalyze the production of this compound.

Andreas Schmid and colleagues from TU Dortmund, Germany, investigated key parameters, such as pathway construction, gene expression, kinetics of the enzymes involved, physiology, reaction engineering and also the bioreactor setup, that influence the production of limonene of genetically modified E. coli. Their comprehensive approach shows that the optimization of such a whole cell process requires thorough analysis of all imaginable processes inside and outside of the microbial cell. By targeted engineering and strain selection they were able to achieve the highest monoterpene concentration ever obtained with a cellular biocatalyst to date.

Article Views: 3455

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

If you would like to reuse any content, in print or online, from ChemistryViews.org, please contact us first for permission and consult our permission guidance prior to making your request

Follow on Facebook Follow on Twitter Follow on YouTube Follow on LinkedIn Follow on Instagram RSS Sign up for newsletters

Magazine of Chemistry Europe (16 European Chemical Societies) published by Wiley-VCH