Re-engineering Cell Walls

Re-engineering Cell Walls

Author: ChemistryViews

Staphylococcus aureus is a Gram-positive bacterial pathogen that has emerged as a major public health threat. Most hospital isolates are resistant to many, if not all, available treatments.

David Spiegel and colleagues, Yale University, USA, demonstrate that the cell surface of wild-type S. aureus can be re-engineered biosynthetically to incorporate non-native small molecules covalently into cell wall peptidoglycan and exploit this biosynthetic labeling technology to perform a bioorthogonal chemical reaction on the S. aureus surface.

The findings were proved through a variety of experimental techniques (e.g., mass spectrometry) as well as an azide-alkyne cycloaddition on the azide incorporated in bacterial cell wall surface.

A diverse range of applications of this strategy can be envisioned: For example, highly selective labeling of bacteria, developing engineered organisms with new functions or for novel therapeutic approaches.

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