The HIV-1 Rev protein is responsible for moving viral mRNA out of cell nuclei and creating new infectious particles of the HIV virus in the body. It is a target for the design of anti-HIV drugs, but its tendency to aggregate has made studying it difficult.
Ashraf Brik and colleagues, Ben-Gurion University of the Negev, Israel, have designed a synthetic route to HIV-1 Rev to better enable its study. The team used a modified native chemical ligation strategy with two ligation reactions: Thz–Cys conversion and a desulfurization step. This could be performed as a one-pot synthesis that gave an isolated yield of 15 %. This method to prepare HIV-1 Rev in high quantity and purity without aggregation is also flexible enough to enable the preparation of new synthetic analogues useful to determine its structure–function relationship.
- Chemical Synthesis and Expression of the HIV-1 Rev Protein
P. Siman, O. Blatt, T. Moyal, T. Danieli, M. Lebendiker, H. A. Lashuel, A. Friedler, A. Brik,