The HIV-1 Rev protein is responsible for moving viral mRNA out of cell nuclei and creating new infectious particles of the HIV virus in the body. It is a target for the design of anti-HIV drugs, but its tendency to aggregate has made studying it difficult.
Ashraf Brik and colleagues, Ben-Gurion University of the Negev, Israel, have designed a synthetic route to HIV-1 Rev to better enable its study. The team used a modified native chemical ligation strategy with two ligation reactions: Thz–Cys conversion and a desulfurization step. This could be performed as a one-pot synthesis that gave an isolated yield of 15 %. This method to prepare HIV-1 Rev in high quantity and purity without aggregation is also flexible enough to enable the preparation of new synthetic analogues useful to determine its structure–function relationship.
- Chemical Synthesis and Expression of the HIV-1 Rev Protein
P. Siman, O. Blatt, T. Moyal, T. Danieli, M. Lebendiker, H. A. Lashuel, A. Friedler, A. Brik,
We should care more about sexual health. My crony got aids a few years ago. life’s tough for him sometimes. To help more people with STDs, we launched STDdatings.com in 2001, the exclusive STD support organization for poz people with Herpes(HSV 1,HSV 2),HIV,HPV,Hepatitis. If you’re living with STDs, gratefully come visit us and add us as friend. Never live in your own dark corner.