Sedimented Solute NMR

Sedimented Solute NMR

Author: ChemViews

β-Amyloid (Aβ) is the main component of certain deposits found in the brains of patients with Alzheimer’s disease.

Claudio Luchinat, Magnetic Resonance Center (CERM), University of Florence, Italy, and colleagues have shown that termed sedimented solutes NMR (SedNMR) allows for collecting and trapping of AβM40 aggregates in a fully hydrated environment without adding cosolvents or interaction partners. Therefore, this provides a unique way to access the formation kinetics and structural features of these species with reduced perturbations.

To immobilize biomolecules and make them amenable for SedNMR studies, they have introduced sedimentation through ultracentrifugation, either by magic angle spinning (in situ) or preparative ultracentrifuge (ex situ). In situ SedNMR was used to address the kinetics of formation of soluble Aβ assemblies by monitoring the disappearance of the monomer and the appearance of the oligomers simultaneously. Ex situ SedNMR allows to select different oligomeric species and to reveal atomic-level structural features of soluble Aβ assemblies.

According to the team this approach can be easily applied to other amyloid systems and, more generally, to the study of many supramolecular assembly processes.

Issue 14/2013 of ChemBioChem is dedicated to the memory of the noted Italian scientist Ivano Bertini, who died in July 2012.


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