Lighting the Way

  • Author: ChemViews
  • Published Date: 31 August 2010
  • Source / Publisher: 3rd EuCheMS Congress, Nuremberg
  • Copyright: Wiley-VCH Verlag GmbH & Co. KGaA
thumbnail image: Lighting the Way

The monitoring of biological systems requires agents that are small enough and similar enough to the system under study that the system is not disturbed during observation. Barbara Imperiali and co-workers, Massachusetts Institute of Technology, USA, have used sulfonamide oxines to monitor phosphorylation in real time.

To achieve high intensities suitable for monitoring, e.g., protein folding, a molecule needs to display a large fluorescence increase on complexation. Generally, this is only achieved with a large molecule. Imperiali has identified a selection of small molecules, including aminophthalimide- and naphthalimide-based compounds, with sufficiently large fluorophores through targeted combinatorial chemistry. She has used these to study the activity of kinases in lung and breast cancer cells, which show 2–3 times higher activity than non-cancer cells.

  • Plenary lecture “Fluorescent Tools for Chemical Biology” given at 3rd EuCheMS Congress, Nuremberg, August 2010.

Article Views: 2991

Sign in Area

Please sign in below

Additional Sign In options

Please note that to comment on an article you must be registered and logged in.
Registration is for free, you may already be registered to receive, e.g., the newsletter. When you register on this website, please ensure you view our terms and conditions. All comments are subject to moderation.

Article Comments - To add a comment please sign in

Bookmark and Share

If you would like to reuse any content, in print or online, from, please contact us first for permission. more

CONNECT: on Facebook on Twitter on YouTube on LinkedIn Sign up for our free newsletter

Magazine of Chemistry Europe (16 European Chemical Societies)published by Wiley-VCH