Halogen Sense

Halogen Sense

Author: David Bradley

By immobilizing purified haloalkane dehalogenase (EC 3.8.1.5) and a fluorescence pH indicator on a fiber-optic tip, researchers in Czech Republic have developed a new type of sensor for halogenated hydrocarbons. They tested the pH sensitivity of various fluorescent dyes and different immobilization conditions in order to optimize the sensor for as low a detection limit as possible.

They demonstrated proof of principle with two well-known organohalogen compounds, 1,2-dibromoethane and 3-chloro-2-(chloromethyl)-1-propene, with detection limits as low as 0.133 and 0.014 mM, respectively.


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